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KMID : 1161420190220090896
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Journal of Medicinal Food
2019 Volume.22 No. 9 p.896 ~ p.906
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Micronized, Heat-Treated Lactobacillus plantarum LM1004 Alleviates Cyclophosphamide-Induced Immune Suppression
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Jung Il-Seon
Jeon Min-Gyu
Oh Da-Som
Jung Ye-Jin
Kim Hyun-Su
Bae Dong-Hyuck
Kim Yu-Jin
Lee Gyeong-Eun
Choi Chul-Yung
Hwang Yong-Pil
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Abstract
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The present study investigated the immunomodulatory activity and associated mechanisms of heat-treated Lactobacillus plantarum LM1004 (HT-LM1004) in a cyclophosphamide (CTX)-induced mouse model of immunosuppression. HT-LM1004 induced phagocytic activity and nitric oxide production in RAW264.7 macrophages and stimulated the release of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-2, and IL-12p70. In mice with CTX-induced immunosuppression, oral HT-LM1004 administration restored thymus and spleen indices, including spleen weight. Consistent with the in vitro results, HT-LM1004 increased TNF-α, IFN-γ, IL-2, and IL-12p70 levels in mice after 14 days of treatment and enhanced the natural killer (NK) cell activity of splenocytes from mice with CTX-induced immunosuppression against YAC-1 lymphoma cells. The method of HT-LM1004 generation influenced this activity: L. plantarum LM1004 grown in a membrane bioreactor, which reduced the size of the cells to <1.0?μm through physical stress (micronization), promoted NK cell cytotoxicity to a greater extent than LM1004 subjected to heat treatment alone. These findings indicate that HT-LM1004 without or with micronization can reverse CTX-induced immunosuppression without adverse side effects by potentiating NK cell function.
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KEYWORD
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cyclophosphamide, cytokine, heat-treated Lactobacillus plantarum LM1004, immune enhancement, macrophage phagocytosis, NK cell activity
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